Descriptively referred to also as massively parallel sequencing (MPS), this high-throughput genomics method developed on various platforms provides genome-scale insights from data for the fields of medical diagnostics, epidemiology, population genetics, and more recently for forensic genetics as well. Next-generation sequencing (NGS) technologies transformed the field of genetics in the past decade.
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The currently available open-source and commercial software for forensic sequencing data analysis are summarized here to provide an accessible starting point for those fairly new to the forensic application of massively parallel sequencing. This review gives an introductory overview for the forensic scientists who are at the beginning of their journey with implementing next-generation sequencing locally and because most in the field do not have a bioinformatics background may find it difficult to navigate the new terms and analysis options available. Despite this, the readily available expertise to analyze, interpret and understand such data is still perceived to be lagging behind.
In the case of next-generation sequencing, in the last decade, several specifically forensic commercial options became available, offering reliable and validated solutions.
The top challenges of adopting new methods to forensic DNA analysis in routine laboratories are often the capital investment and the expertise required to implement and validate such methods locally.
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